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Catalog No: OKCD00801 (Formerly GWB-KBBCI2)
Size:96WELLS
Price: $650.00
SKU
OKCD00801
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Datasheets/ManualsPrintable datasheet for PTH2R ELISA Kit (Human) (OKCD00801)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parathyroid Hormone Receptor 2 (PTHR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parathyroid Hormone Receptor 2 (PTHR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parathyroid Hormone Receptor 2 (PTHR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Parathyroid Hormone Receptor 2 (PTHR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Receptor 2 (PTHR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Receptor 2 (PTHR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PTH2R Microplate96 Wells (12 x 8 Well Strips)
PTH2R Lyophilized Standard2 x 20 ng
100X Biotinylated PTH2R Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone Receptor 2 (PTHR2). No significant cross-reactivity or interference between Parathyroid Hormone Receptor 2 (PTHR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPTH2R
Gene Full Nameparathyroid hormone 2 receptor
Alias Symbolsparathyroid hormone 2 receptor;parathyroid hormone receptor 2;PTH2 receptor;PTHR2.
NCBI Gene Id5746
Protein NameParathyroid hormone 2 receptor
Description of TargetThis is a specific receptor for parathyroid hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. PTH2R may be responsible for PTH effects in a number of physiological systems. It may play a significant role in pancreatic function. PTH2R presence in neurons indicates that it may function as a neurotransmitter receptor (By similarity).
Uniprot IDP49190
Protein Accession #NP_001296445
Nucleotide Accession #NM_001309516.1
Protein Size (# AA)550
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