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Catalog No: OKEH02377
Size:96T
Price: $625.00
SKU
OKEH02377
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PTEN ELISA Kit (Human) (OKEH02377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTEN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTEN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTEN captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
PTEN Microplate96 Wells (12 x 8 Well strips)
PTEN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated RAB8A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolPTEN
Gene Full Namephosphatase and tensin homolog
Alias Symbols10q23del;BZS;CWS1;DEC;GLM2;MHAM;mitochondrial phosphatase and tensin protein alpha;mitochondrial PTENalpha;MMAC1;MMAC1 phosphatase and tensin homolog deleted on chromosome 10;mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;phosphatase and tensin-like protein;phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;protein tyrosine phosphatase;PTEN variant PTEN-K267Rfs*9;PTEN variant PTEN-L247*;PTEN variant PTEN-R234Afs*11;PTEN1;PTENbeta;PTENepsilon;TEP1.
NCBI Gene Id5728
Protein NameP60484
Description of TargetPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Uniprot IDhttps://www.uniprot.org/uniprot/P60484
Protein Accession #NP_000305.3
Nucleotide Accession #NM_000314.6
Protein Size (# AA)403
Molecular Weight47 kDa
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