PSMF1 Antibody (OAGA00344)

Catalog No: OAGA00344

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
OAGA00344 diluted at 1:1000

PSMF1 antibody [C2C3], C-term detects PSMF1 protein at Cytoplasm and Nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSMF1 protein stained by PSMF1 antibody [C2C3], C-term (OAGA00344) diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq]
  Positive Controls: HepG2
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 209 and 271 of PSMF1 (Uniprot ID#Q92530)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PSMF1
• Gene Full Name: Proteasome inhibitor PI31 subunit
• Alias Symbols: PI31
• NCBI Gene Id: 9491
• Protein Name: Proteasome inhibitor PI31 subunit
• Uniprot ID: Q92530
• Protein Accession #: NP_006805.2
• Molecular Weight: 30 kDa