PSME3 Antibody (OAGA00529)

Catalog No: OAGA00529

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
OAGA00529 diluted at 1:2000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
12% SDS PAGE
OAGA00529 diluted at 1:2000

Sample (30 ug of whole cell lysate)
A: PC-12
12% SDS PAGE
OAGA00529 diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3(OAGA00529) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3(OAGA00529) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]
  Positive Controls: A549, H1299, HCT116, MCF-7, NIH-3T3, JC, BCL-1, PC-12
  Cellular Localization: Nucleus, Cytoplasm (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.65 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PSME3
• Gene Full Name: Proteasome activator complex subunit 3
• Alias Symbols: Ki, PA28G, HEL-S-283, PA28gamma, REG-GAMMA, PA28-gamma
• NCBI Gene Id: 10197
• Protein Name: Proteasome activator complex subunit 3
• Uniprot ID: P61289
• Protein Accession #: NP_005780.2
• Molecular Weight: 30 kDa