PSMB8 Antibody (OAGA01026)

Catalog No: OAGA01026

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
OAGA01026 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSMB8(OAGA01026) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMB8(OAGA01026) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq]
  Positive Controls: A431, Molt-4, Raji
  Cellular Localization: Cytoplasm (By similarity), Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 52 and 276 of PSMB8 (Uniprot ID#P28062)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.38 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PSMB8
• Gene Full Name: Proteasome subunit beta type-8
• Alias Symbols: JMP, ALDD, LMP7, NKJO, D6S216, PRAAS1, PSMB5i, RING10, D6S216E
• NCBI Gene Id: 5696
• Protein Name: Proteasome subunit beta type-8
• Uniprot ID: P28062
• Protein Accession #: NP_004150.1
• Molecular Weight: 30 kDa