PSMB6 ELISA Kit (Human) (OKCD00993)

Catalog No: OKCD00993 (Formerly GWB-KBBTW4)

Size:96WELLS

Human PSMB6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Beta Type 6 (PSMb6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Beta Type 6 (PSMb6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Beta Type 6 (PSMb6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proteasome Subunit Beta Type 6 (PSMb6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteasome Subunit Beta Type 6 (PSMb6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteasome Subunit Beta Type 6 (PSMb6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PSMB6 Microplate: 96 Wells (12 x 8 Well Strips)
  • PSMB6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PSMB6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Beta Type 6 (PSMb6). No significant cross-reactivity or interference between Proteasome Subunit Beta Type 6 (PSMb6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PSMB6
• Gene Full Name: proteasome 20S subunit beta 6
• Alias Symbols: DELTA;LMPY;macropain delta chain;multicatalytic endopeptidase complex delta chain;proteasome (prosome, macropain) subunit, beta type, 6;proteasome catalytic subunit 1;proteasome delta chain;proteasome subunit beta 6;proteasome subunit beta type-6;proteasome subunit beta1;proteasome subunit delta;proteasome subunit Y;PSY large multifunctional protease Y;Y.
• NCBI Gene Id: 5694
• Protein Name: Proteasome subunit beta type-6
• Description of Target: Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.
• Uniprot ID: P28072
• Protein Accession #: NP_002789
• Nucleotide Accession #: NM_002798.2
• Protein Size (# AA): 239