PSMA7 Antibody (OAGA01025)

Catalog No: OAGA01025

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
12% SDS PAGE
OAGA01025 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
OAGA01025 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using proteasome alpha 7(OAGA01025) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Cytoplasm, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 205 of Proteasome 20S alpha 7 (Uniprot ID#O14818)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.86 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PSMA7
• Gene Full Name: Proteasome subunit alpha type-7
• Alias Symbols: C6, HSPC, RC6-1, XAPC7, HEL-S-276
• NCBI Gene Id: 5688
• Protein Name: Proteasome subunit alpha type-7
• Uniprot ID: O14818
• Protein Accession #: NP_002783.1
• Molecular Weight: 28 kDa