PSG1 ELISA Kit (Human) (OKEH04019)

Catalog No: OKEH04019

Size:96T

Human PSG1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PSG1 ELISA Kit (Human) (OKEH04019) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PSG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PSG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PSG1 captured in the well.
• ELISA Kit Range: 15.6-1000ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 3.8% (n = 20)
  Mean Inter-assay CV%: <= 5.9% (n = 20)
• ELISA Kit Component:
  • Anti-PSG1 Microplate: 96 Wells (12 x 8 Well Strips)
  • PSG1 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • PSG1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 10 ng/mL
• Specificity: Natural and recombinant Human Pregnancy-specific beta-1-glycoprotein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PSG1
• Gene Full Name: pregnancy specific beta-1-glycoprotein 1
• Alias Symbols: B1G1;CD66 antigen-like family member F;CD66f;DHFRP2;fetal liver non-specific cross-reactive antigen 1/2;FL-NCA-1/2;PBG1;pregnancy-specific B-1 glycoprotein;pregnancy-specific beta-1 glycoprotein C/D;pregnancy-specific beta-1-glycoprotein 1;PS-beta-C/D;PS-beta-G-1;PSBG1;PSBG-1;PSG95;PSGGA;PSGIIA;SP1.
• NCBI Gene Id: 5669
• Protein Name: P11464
• Description of Target: Pregnancy-specific beta-1-glycoprotein 1
• Uniprot ID: https://www.uniprot.org/uniprot/P11464
• Protein Accession #: NP_001171754.1
• Nucleotide Accession #: NM_001184825.1
• Protein Size (# AA): 419
• Molecular Weight: 47 kDa