PRTN3 ELISA Kit (Mouse) (OKCD02819)

Catalog No: OKCD02819

Size:96WELLS

Mouse PRTN3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	84-92%	98-105%	97-105%
  EDTA Plasma (n=5)	95-103%	80-99%	79-93%	80-96%
  Heparin Plasma (n=5)	86-99%	85-99%	93-101%	78-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteinase 3 (PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteinase 3 (PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteinase 3 (PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proteinase 3 (PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-101	90
  EDTA Plasma (n=5)	80-90	86
  Heparin Plasma(n=5)	82-90	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteinase 3 (PR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteinase 3 (PR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PRTN3 Microplate: 96 Wells (12 x 8 Well strips)
  • PRTN3 Lyophilized Standard: 2
  • 100X Biotinylated PRTN3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Proteinase 3 (PR3). No significant cross-reactivity or interference between Proteinase 3 (PR3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Prtn3
• Gene Full Name: proteinase 3
• Alias Symbols: mP;mPR3;myeloblastin;P;PR3;PR-3;Proteinase 3.
• NCBI Gene Id: 19152
• Protein Name: Myeloblastin
• Description of Target: Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.
• Uniprot ID: Q61096
• Protein Accession #: NP_035308.2
• Nucleotide Accession #: NM_011178.2
• Protein Size (# AA): 254