PROS1 ELISA Kit (Rat) (OKEH03431)

Catalog No: OKEH03431

Size:96T

Rat PROS1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PROS1 ELISA Kit (Rat) (OKEH03431) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Pros1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pros1 and incubated. The Pros1 found in the sample or standards competes with the biotinylated Pros1 for limited binding sites on the immobilized anti-Rat Pros1 antibody. Excess unbound biotinylated Pros1 and sample or standard Pros1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pros1 captured in the well and inversely proportional to the amount of Pros1 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Component:
  • Anti-Pros1 Microplate: 96 Wells (12 x 8 Well strips)
  • Pros1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Pros1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.781 ng/mL
• Specificity: Natural and recombinant Rat Vitamin K-dependent protein S
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PROS1
• Gene Full Name: protein S (alpha)
• Alias Symbols: Pros
• NCBI Gene Id: 81750
• Protein Name: P53813
• Description of Target: Vitamin K-dependent protein S
• Uniprot ID: https://www.uniprot.org/uniprot/P53813
• Protein Accession #: NP_112348.2
• Nucleotide Accession #: NM_031086.2
• Protein Size (# AA): 675
• Molecular Weight: 75 kDa