Catalog No: OKEH01402
Size:96T
Price: $525.00
SKU
OKEH01402
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PROS1 ELISA Kit (Human) (OKEH01402) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PROS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PROS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PROS1 captured in well.
ELISA Kit Range6.25-400ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
PROS1 Microplate96 Wells (12 x 8 Well strips)
PROS1 Lyophilized Standard2
100X Biotinylated PROS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.56 ng/mL
SpecificityNatural and recombinant Human Vitamin K-dependent protein S
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPROS1
Gene Full Nameprotein S
Alias SymbolsPROS;protein S (alpha);protein Sa;PS21;PS22;PS23;PS24;PS25;PSA;THPH5;THPH6;vitamin K-dependent plasma protein S;vitamin K-dependent protein S.
NCBI Gene Id5627
Protein NameP07225
Description of TargetVitamin K-dependent protein S
Uniprot IDhttps://www.uniprot.org/uniprot/P07225
Protein Accession #NP_000304.2
Nucleotide Accession #NM_000313.3
Protein Size (# AA)676
Molecular Weight75 kDa
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