Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross reactivity to Human Serum Proteins (1 mg)

Catalog#: OAPB01379

• Size: 1 mg

• Host: Goat
• Application: ELISA, WB, IHC
• Conjugation: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Immunogen: Mouse IgG whole molecule
• Purification: Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Applications Info: Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:2,000 - 1:8,000, Western Blot 1:500 - 1:4,000, Immunohistochemistry 1:500 - 1:2,000. (Optimal dilution should be determined by user.)
• Additional Information: Preparation: PHYSICAL STATE: Liquid (sterile filtered) ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)
  Buffer: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Reconstitution and Storage: Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.