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5ug
$100.00
In Stock

PRL R Rabbit - Recombinant Rabbit Prolactin Soluble Receptor (5ug)

Product Introduction: Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Product Description: Prolactin Receptor Rabbit Extra Celleular Domain Recombinant *produced in E.Coli is a non-glycosylated, Polypeptide chain containing 207 amino acids and having a molecular mass of 23972 Dalton. The Prolactin Receptor is purified by proprietary chromatographic techniques.
Description of Target:
Recombinant Rabbit Prolactin Soluble Receptor
Gene Symbol:
raPrl R
NCBI Gene Id:
100009046
Alias Symbols:
PRL-R; PRL R Rabbit; Prolactin Rabbit Soluble Receptor Recombinant
Protein Accession #:
NP_001075700.1
Host:
E. Coli
Protein Name:
Prolactin receptor
Product Format:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity:
Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Peptide Sequence:
The sequence of the first five N - terminal amino acids was determined and was found to be Gly - Lys - Pro - Phe - Ile.
Additional Information:
Solubility: It is recommended to reconstitute the lyophilized PRL-R in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
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Biological Activity: Activity is determined by the dose-dependant inhibition of Prolactin-stimuled proliferation of Nb2 cells and by high affinity binding of oPLR and other lactogenic hormones.
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Protein Content: UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).

Protein Content: UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).
Reconstitution and Storage:
Lyophilized PRL-R although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Prolactin Receptor should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
Datasheets / Downloads:
OPPA00533
 
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