Biotinylated CXCL8 Protein (OPCB00013)

Catalog No: OPCB00013

Size:2ug

Chemokine Induced Migration Cells expressing recombinant CXCR1 were assayed for migration through the transwell bare filter at various concentrations of IL-8. The response is expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

CXCL8-Biotin Detection on PAGE Gel Detection of various amounts (1-62 ng) of Biotinylated-CXCL8 with streptavidin conjugated to 800CW dye (LiCOR).

Product Info

• Product Format: Lyophilized
• Conjugation: Biotin
• Application: CA, SDS-PAGE
• Additional Information: Extinction Coefficient: 12,900 M-1 cm-1
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method.
• Reconstitution and Storage: Spin tube prior to resuspending. Reconstitution is recommended at 100 ug/mL in sterile water. Stable at least 1 month at -20C to -70C under sterile conditions after reconstitution; 12 months from date of receipt, -20C to -70C as supplied. Suggested to use immediately after reconstitution. Avoid repeated freeze/thaw cycles.
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 0.5-1nM determined by Migration Assay in cells expressing recombinant CXCR1
• Source: E. coli
• Reference: 1. "Interleukin-8, a chemotactic and inflammatory cytokine"
  Baggiolini M., Clark-Lewis I.
  FEBS Lett. 307:97-101(1992)
  2. "Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction MDNCF mRNA by interleukin 1 and tumor necrosis factor"
  Matsushima K., Morishita K., Yoshimura T., Lavu S., Kobayashi Y., Lew W., Appella E., Kung H., Leonard E.J., Oppenheim J.J.
  J. Exp. Med. 167:1883-1893(1988)
  3. "Chemokines, CXC|IL-8"
  Strieter R.M., Keane M.P., Belperio J. A.
  Encyclopedia of respiratory medicine, Academic Press, Oxford, P395-398(2006)

Target Info

• Gene Symbol: CXCL8
• Gene Full Name: chemokine (C-X-C motif) ligand 8
• Alias Symbols: IL8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, SCYB8
• NCBI Gene Id: 3576
• Description of Target: Interleukin 8 (IL-8 )(CXCL8) is secreted primarily by macrophages and monocytes. It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for newtrophiles and monoccytes, and promotes activation of these target cells by binding to two cell surface receptors CXCR1 and CXCR2. It is also a strong angiogenic agent, and is considered to play a role in the pathogenesis of bronchiolitis. Biotinylated CXCL8 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CXCL8 in migration assay. Utilizing avidin/streptavidin analogues conjugated to various fluorescent labels, biotinylated CXCL8 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and can replace radioactively labeled chemokines.
• Uniprot ID: P10145
• Molecular Weight: 11 kDa