Biotinylated CCL19 Protein (OPCB00021)

Catalog No: OPCB00021

Size:2ug

Migration Assay: Cells expressing recombinant CCR7 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-3beta. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Product Info

• Product Format: Lyophilized.
• Conjugation: Biotin
• Application: CA
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method
  Extinction Coefficient: 14,180 M-1 cm-1
• Reconstitution and Storage: Spin tube prior to resuspending. Recommended at 100 ug/mL in sterile water
  Store as supplied, 12 months from date of receipt at -20 to -70°C. At least 1 month from reconstitution at -20 to -70°C. Recommended to use immediately after reconstitution.
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 15-30 nM determined by migration assay with cells expressing recombinant CCR7
  
• Source: E. coli
• Reference: 1. "Molecular Cloning of a Novel Human CC Chemokine EBI1-ligand Chemokine That Is a Specific Functional Ligand for EBI1, CCR7"
  Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O.
  J Biol Chem 272:13803-13809 (1997)
  
  2. "CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells."
  Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K., Broxmeyer H.E.
  J. Immunol. 160:2418-2424(1998)
  
  3. "Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication."
  Damås J.K., Landrø L., Fevang B., Heggelund L., Tjønnfjord G.E., Fløisand Y., Halvorsen B., Frøland S.S., Aukrust P.
  Clin Exp Immunol. 157:400-7 (2009)

Target Info

• Gene Symbol: CCL19
• Gene Full Name: chemokine (C-C motif) ligand 19
• Alias Symbols: ELC, CKb11, MIP3B, MIP-3b, SCYA19
• NCBI Gene Id: 6363
• Description of Target: Macrophage inflammatory protein-3-beta (MIP3-beta) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constituitively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. Being a homeostatic chemokine, its primary physiological role is considered to be in the normal recirculation and homing of lymphocyte. However, MIP3-beta could also be proinfammatory, and is implicated in the post-HIV infection responses.
• Uniprot ID: Q99731
• Molecular Weight: 11 kDa