Biotinylated CCL14 Protein (OPCB00022)

Catalog No: OPCB00022

Size:2ug

Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated HCC-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Product Info

• Product Format: Lyophilized
• Conjugation: Biotin
• Application: CA
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method
• Reconstitution and Storage: Recommended at 100ug/mL in sterile water
  Shipping: Room Temp
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 0.2-0.4 nM determined by migration assay with cells expressing recombinant CCR1
  
• Source: E. coli
• Reference: 1. "HCC-1, a novel chemokine from human plasma"
  Schulz-Knappe P., Mägert H., Dewald B., etc
  J Exp Med 183:295-299 (1996)
  2. "Urokinase Plasminogen Activator and Plasmin Efficiently Convert Hemofiltrate CC Chemokine 1 into Its Active [9–74] Processed Variant"
  Vakili J., Standker L., Detheux M., Vassart, G., Forssmann W., Parmentier M
  J Immunol 167:3406-3413 (2001)
  

Target Info

• Gene Symbol: CCL14
• Gene Full Name: chemokine (C-C motif) ligand 14
• Alias Symbols: CC-1, CC-3, CKB1, MCIF, NCC2, SY14, HCC-1, HCC-3, NCC-2, SCYL2, SCYA14, HCC-1(1-74), HCC-1/HCC-3
• NCBI Gene Id: 6358
• Description of Target: Hemofiltrate CC chemokine-1(HCC-1)(CCL14) is constitutively expressed by numerous tissues. Upon processing of the N terminal residues of the full length HCC-1 by the uPA-plasmin system, the active form of HCC-1 is a strong agonist for CCR1, CCR5 and to a lesser extent CCR3, and causes chemotaxis of different types of leukocytes. The active form of HCC-1 is also shown as a potent inhibitor of HIV entry.
• Uniprot ID: Q16627