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Datasheets/Manuals | Printable datasheet for anti-CDH1 (ARP51289_P050) antibody |
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Tested Species Reactivity | Human, Rat |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | IF, WB, IHC |
Additional Information | IHC Information: Breast IHC Information: Liver |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human CDH1 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 79%; Horse: 93%; Human: 100%; Mouse: 79%; Pig: 93%; Rabbit: 93%; Rat: 93%; Zebrafish: 85% |
Peptide Sequence | Synthetic peptide located within the following region: QEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDRED |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-CDH1 (ARP51289_P050) antibody is Catalog # AAP51289 (Previous Catalog # AAPS22404) |
Reference | Onder,T.T., (2008) Cancer Res. 68 (10), 3645-3654 |
Gene Symbol | CDH1 |
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Gene Full Name | Cadherin 1, type 1, E-cadherin (epithelial) |
Alias Symbols | UVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324 |
NCBI Gene Id | 999 |
Protein Name | Cadherin-1 |
Description of Target | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Uniprot ID | P12830 |
Protein Accession # | NP_004351 |
Nucleotide Accession # | NM_004360 |
Protein Size (# AA) | 882 |
Molecular Weight | 80kDa |
Protein Interactions | UBC; MDM2; UCHL3; CDC27; PTEN; CTNNB1; PIP5K1C; CTNND1; CBLL1; RNF2; GMNN; LMNA; KRT18; KRT9; KRT8; KRT1; IGHA1; HSPD1; HSP90AA1; HSPA9; HSPA8; HSPA5; GBAS; CTNNA1; CRYAB; CFTR; C1QBP; ACTN4; ACADVL; ANP32B; NIPSNAP1; XRCC5; TGM2; SKP2; SET; RAD17; POLR2E |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CDH1 Antibody - middle region (ARP51289_P050)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish".
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How long will it take to receive "CDH1 Antibody - middle region (ARP51289_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "CDH1 Antibody - middle region (ARP51289_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CDH1 Antibody - middle region (ARP51289_P050)"?
This target may also be called "UVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324" in publications.
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What is the shipping cost for "CDH1 Antibody - middle region (ARP51289_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CDH1 Antibody - middle region (ARP51289_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CDH1 Antibody - middle region (ARP51289_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "80kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CDH1 Antibody - middle region (ARP51289_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CDH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CDH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CDH1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CDH1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CDH1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CDH1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.