IkappaB-epsilon Antibody (Phospho-Ser22) (OAAF07320)

Catalog No: OAAF07320 (Formerly GWB-ASB015)

Size:100 ug

Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30', using IkappaB-epsilon (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Modification Sites: Human:S22 Mouse:S22 Rat:S22
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Peptide Sequence: Synthetic peptide located within the following region: GPRPARAAGMSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPS
• Concentration: 1mg/ml
• Specificity: IkappaB-epsilon (Phospho-Ser22) Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at Ser22.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  IF: 1:100~1:500
  ELISA: 1:40000

Target Info

• Gene Symbol: NFKBIE
• Gene Full Name: NFKB inhibitor epsilon
• Alias Symbols: ikappaBepsilon;I-kappa-B-epsilon;ikB-E;IKBE;ikB-epsilon;NF-kappa-B inhibitor epsilon;NF-kappa-BIE;nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon.
• NCBI Gene Id: 4794
• Protein Name: NF-kappa-B inhibitor epsilon
• Description of Target: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
• Uniprot ID: O00221
• Molecular Weight: 38 kDa