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Catalog No: OAPC00074 (Formerly GWB-B37AB9)
Size:100 ul
Price: $390.00
SKU
OAPC00074
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsPrintable datasheet for OAPC00074
Product Info
Predicted Species ReactivityHuman, Rat
Product FormatLiquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
ClonalityPolyclonal
HostSheep
ApplicationWB, IHC
Additional InformationPackaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and StorageFor long term storage -20C is recommended. Stable at -20C for at least 1 year.
ImmunogenRecombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
PurificationPrepared from sheep serum by affinity purification using a column to which the recombinant protein was coupled.
Predicted Homology Based on Immunogen SequenceHuman; Rat
SpecificitySpecific for the ~55k tryptophan hydroxylase protein.
Application InfoThe antibody has been directly tested for reactivity in Western blots with human and rat tissue. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in rabbit tissues.
Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.
ReferenceHaycock JW, Kumer SC, Lewis DA, Vrana KE, Stockmeier CA (2002) A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. J Neurosci Methods 114:205-212.
Martinez A, Knappskog PM, Haavik J (2001) A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
Gene SymbolTPH1
Alias SymbolsTPH
NCBI Gene Id100101558
Protein NameTryptophan 5-hydroxylase 1
Description of TargetTryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002). Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).
Uniprot IDP17290
Protein Accession #NP_001075741.1
Molecular Weight~55kDa
  1. What is the species homology for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Rat".

  2. How long will it take to receive "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Anti-Tryptophan Hydroxylase (OAPC00074)" provided in?

    This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    This target may also be called "TPH" in publications.

  5. What is the shipping cost for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~55kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Anti-Tryptophan Hydroxylase (OAPC00074)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "TPH1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "TPH1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "TPH1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "TPH1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "TPH1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "TPH1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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