Anti-Synapsin I (OAPC00062)

Catalog No: OAPC00062 (Formerly GWB-353D30)

Size:10 ug

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.

Immunostaining of cultured rat caudate neurons showing punctate distribution of synapsin in green and MAP in red. Cells and photo courtesy of QBMCellScience.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized 5mM ammonium bicarbonate. Adequate amount of material to conduct 10 mini Western blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IF, IP, IHC
• Reconstitution and Storage: The lyophilized antibody can be stored at -20°C and is stable for at least 2 years. The antibody should be reconstituted in 50 uL PBS (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4 pH 7.4) before use. After reconstitution, the antibody should be aliquoted and stored at -20°C.
• Immunogen: Native protein purified from bovine brain.
• Purification: Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.
• Specificity: Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Application Info: DB: 1:1000
  IHC: 1:2000
  IF: 1:2000
  IP: 1 ug per 200 ug lysate
  WB: 1:1000
• Reference: Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.
  Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.
  Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.
  Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
  Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.
  Stone LM, Browning MD, Finger TE (1994) Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci 14:301-309.
  Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not alpha -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 - 33146.
  Note: Dr. Michael Browning, co-author of the cited papers is the President and founder PhosphoSolutions.

Target Info

• Gene Symbol: SYN1
• NCBI Gene Id: 281510
• Protein Name: Synapsin-1
• Description of Target: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
• Uniprot ID: P17599
• Protein Accession #: NP_776616.1
• Molecular Weight: ~78kDa