Anti-NMDA Receptor, NR2C Subunit (OAPC00036)

Catalog No: OAPC00036 (Formerly GWB-4867A0)

Size:10 ug

Western blot of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor.

Immunostaining of rabbit retina showing NR2C punctuate distribution in the center of the inner plexiform layer.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquoted and
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IP, IHC, IF
• Additional Information: Packaging :
  10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquoted and stored at -20oC. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year.
• Immunogen: Fusion protein from the N-terminus of the NR2C-subunit of rat NMDA receptor.
• Purification: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: Specific for the ~140k NR2C subunit of the NMDA receptor. Also labels the ~180k NR2A and the ~180k NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.
• Application Info: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
  Immunostaining of rabbit retina showing NR2C punctuate distribution in the center of the inner plexiform layer.
  Western blot of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor.
• Reference: Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
  Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
  Ebralidze AK, Rossi DJ, Tonegawa S, Slater NT (1996) Modification of NMDA receptor channels and synaptic transmission by targeted disruption of the NR2C gene. J Neurosci 16:5014-5025.
  Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
  Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.
  Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.
  Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78.
  Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.
  Note: Dr. Michael Browning, a co-author of four of the cited papers, is President and founder of PhosphoSolutions.

Target Info

• Gene Symbol: GRIN2C
• Alias Symbols: NR2C, GluN2C
• NCBI Gene Id: 24411
• Protein Name: Glutamate receptor ionotropic, NMDA 2C
• Description of Target: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996).
• Uniprot ID: Q00961
• Protein Accession #: NP_036707.3
• Molecular Weight: ~140kDa