Anti-Dopamine Transporter, Extracellular Loop 2 (OAPC00105)

Catalog No: OAPC00105 (Formerly GWB-84BACA)

Size:100 ul

Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year.
• Immunogen: Peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).
• Purification: Prepared from rabbit serum by affinity purification using a SulfoLink column matrix to which the peptide immunogen was coupled.
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: Specific for the ~88k DAT protein in Western blots.
• Application Info: The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.
  Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.
  
• Reference: Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
  Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.
  Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
  Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

Target Info

• Gene Symbol: SLC6A3
• Alias Symbols: DAT, DAT1, PKDYS, PKDYS1
• NCBI Gene Id: 6531
• Protein Name: Sodium-dependent dopamine transporter
• Description of Target: The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
• Uniprot ID: Q01959
• Protein Accession #: NP_001035.1
• Molecular Weight: ~88kDa