- Gene Symbol:
- NCBI Gene Id:
- Official Gene Full Name:
- Signal transducer and activator of transcription 3 (acute-phase response factor)
- Protein Name:
- Signal transducer and activator of transcription 3
- Swissprot Id:
- Protein Accession #:
- Nucleotide Accession #:
- Alias Symbols:
- APRF, FLJ20882, MGC16063, HIES
- Replacement Item:
- This antibody may replace item sc-126054 from Santa Cruz Biotechnology.
- Description of Target:
- STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act a
- Protein Size (# AA):
- Molecular Weight:
- Affinity Purified
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express STAT3.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express STAT3.
- The immunogen is a synthetic peptide directed towards the middle region of human STAT3
- Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
- Predicted Homology Based on Immunogen Sequence:
- Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 91%
- Complete computational species homology data:
- Anti-STAT3 (ARP30317_P050)
- Peptide Sequence:
- Synthetic peptide located within the following region: SNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATS
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Protein Interactions:
- MTOR; GADD45A; BMX; BLK; UBC; SYK; LMO2; SRI; HIC1; GNB2; USP22; RELA; COPS5; BRD4; ADRB2; PSMG3; TBCD; TBC1D15; PUS7; LUC7L2; ARFIP1; IVNS1ABP; MSH2; BMH1; SIN3A; EGFR; PIAS3; HDAC3; SRC; LYN; JAK3; JAK2; HES5; TRIM28; HES1; HCK; FYN; FGR; HNRNPM; SULT2A
- Blocking Peptide:
- For anti-STAT3 (ARP30317_P050) antibody is Catalog # AAP30317 (Previous Catalog # AAPS08804)
- Datasheets / Downloads:
- Printable datasheet for anti-STAT3 (ARP30317_P050) antibody
- Target Reference:
- Ohbayashi,N., (2008) Biochem. Biophys. Res. Commun. 371 (4), 823-828
Aviva Systems Biology is the original manufacturer of this STAT3 antibody (ARP30317_P050)
Click here to view the STAT3 antibody Western Blot Protocol
Product Datasheet Link: STAT3 antibody (ARP30317_P050)
WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: THP-1
Western Blot image:
Description of Target: STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act a
Questions pertaining to this data can be directed to firstname.lastname@example.org
Aviva Systems Biology’s STAT3 antibody (ARP30317_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at email@example.com.
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Aviva Systems Biology is the original manufacturer of this STAT3 antibody.
Product Datasheet Link: STAT3 antibody ARP30317_P050
Rabbit Anti-STAT3 Antibody
Catalog Number: ARP30317_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Exposure Time: 0.5 - 2.0 sec
Left to right:
DAPI, STAT3 Ab, Merge
Control Antibody: Normal Rabbit IgG
Control Antibody Concentration: 1:100
Left to right:
DAPI, Rabbit IgG, Merge
1. Normal adult human lung tissue was formalin fixed, embedded in paraffin wax, sectioned at 6 micron thickness and put on histological slides.
2. After deparaffinization and rehydration, the low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
3. The blocking buffer was 5% normal goat serum.
4. Primary antibodies was diluted in antibody dilution buffer (1% Normal Donkey Serum) to the final testing dilution (1:100, 1:600, 1:1200).
5. The appropriate anti-rabbit fluorescent-conjugated (Rhodamine:red or FITC:green) secondary antibody was applied and nuclei will be counterstained with DAPI (blue).
6. A Negative control utilized a nonspecific rabbit IgG staining the same normal adult human lung tissue.