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STAT3 antibody - middle region (ARP30317_P050)

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100 ul
$289.00
In Stock

Conjugation Options

ARP30317_P050-FITC Conjugated

ARP30317_P050-HRP Conjugated

ARP30317_P050-Biotin Conjugated

Gene Symbol:
STAT3
NCBI Gene Id:
6774
Official Gene Full Name:
Signal transducer and activator of transcription 3 (acute-phase response factor)
Protein Name:
Signal transducer and activator of transcription 3
Swissprot Id:
P40763
Protein Accession #:
NP_644805
Nucleotide Accession #:
NM_139276
Alias Symbols:
APRF, FLJ20882, MGC16063, HIES
Replacement Item:
This antibody may replace item sc-126054 from Santa Cruz Biotechnology.
Description of Target:
STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act a
Protein Size (# AA):
770
Molecular Weight:
88kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express STAT3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express STAT3.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human STAT3
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 91%
Complete computational species homology data:
Anti-STAT3 (ARP30317_P050)
Peptide Sequence:
Synthetic peptide located within the following region: SNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATS
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
MTOR; GADD45A; BMX; BLK; UBC; SYK; LMO2; SRI; HIC1; GNB2; USP22; RELA; COPS5; BRD4; ADRB2; PSMG3; TBCD; TBC1D15; PUS7; LUC7L2; ARFIP1; IVNS1ABP; MSH2; BMH1; SIN3A; EGFR; PIAS3; HDAC3; SRC; LYN; JAK3; JAK2; HES5; TRIM28; HES1; HCK; FYN; FGR; HNRNPM; SULT2A
Blocking Peptide:
For anti-STAT3 (ARP30317_P050) antibody is Catalog # AAP30317 (Previous Catalog # AAPS08804)
Datasheets / Downloads:
Printable datasheet for anti-STAT3 (ARP30317_P050) antibody
Target Reference:
Ohbayashi,N., (2008) Biochem. Biophys. Res. Commun. 371 (4), 823-828

Product Protocols: STAT3 antibody tested with Human Thp-1 Cells (ARP30317_P050)

Aviva Systems Biology is the original manufacturer of this STAT3 antibody (ARP30317_P050)

Click here to view the STAT3 antibody Western Blot Protocol

Product Datasheet Link: STAT3 antibody (ARP30317_P050)

WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: THP-1

Western Blot image:


Description of Target: STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act a

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s STAT3 antibody (ARP30317_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: STAT3 Antibody (ARP30317_P050) in Human Lung Tissue using Immunohistochemistry

Aviva Systems Biology is the original manufacturer of this STAT3 antibody.
Product Datasheet Link: STAT3 antibody ARP30317_P050

Rabbit Anti-STAT3 Antibody
Catalog Number: ARP30317_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Left to right:
DAPI, STAT3 Ab, Merge

Control Image:
Control Antibody: Normal Rabbit IgG
Control Antibody Concentration: 1:100

Left to right:
DAPI, Rabbit IgG, Merge

Protocol:
1. Normal adult human lung tissue was formalin fixed, embedded in paraffin wax, sectioned at 6 micron thickness and put on histological slides.
2. After deparaffinization and rehydration, the low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
3. The blocking buffer was 5% normal goat serum.
4. Primary antibodies was diluted in antibody dilution buffer (1% Normal Donkey Serum) to the final testing dilution (1:100, 1:600, 1:1200).
5. The appropriate anti-rabbit fluorescent-conjugated (Rhodamine:red or FITC:green) secondary antibody was applied and nuclei will be counterstained with DAPI (blue).
6. A Negative control utilized a nonspecific rabbit IgG staining the same normal adult human lung tissue.

 
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