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DFFA antibody - N-terminal region (ARP30359_T100)

  • Catalog#: ARP30359_T100
  • Domestic: within 1-2 days delivery International: 1-2 days

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    - Trial Size Available. Trial size is not available for conjugation.
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100 ul
    $229.00
    In Stock

    Conjugation Options

    ARP30359_T100-FITC Conjugated

    ARP30359_T100-HRP Conjugated

    ARP30359_T100-Biotin Conjugated

    Free trial-size samples may be available for this item. Please go here for more information.
    Gene Symbol:
    DFFA
    NCBI Gene Id:
    1676
    Official Gene Full Name:
    DNA fragmentation factor, 45kDa, alpha polypeptide
    Protein Name:
    DNA fragmentation factor subunit alpha
    Swissprot Id:
    O00273
    Protein Accession #:
    NP_004392
    Nucleotide Accession #:
    NM_004401
    Alias Symbols:
    DFF-45, DFF1, Ion ChannelAD, ICAD
    Replacement Item:
    This antibody may replace item sc-17816 from Santa Cruz Biotechnology.
    Description of Target:
    Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
    Protein Size (# AA):
    331
    Molecular Weight:
    37kDa
    Host:
    Rabbit
    Clonality:
    Polyclonal
    Purification:
    Protein A purified
    Application:
    WB
    Tissue Tool:
    Find tissues and cell lines supported by DNA array analysis to express DFFA.
    RNA Seq:
    Find tissues and cell lines supported by RNA-seq analysis to express DFFA.
    Immunogen:
    The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA
    Species Reactivity:
    Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
    Predicted Homology Based on Immunogen Sequence:
    Cow: 92%; Dog: 83%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 83%; Rat: 92%
    Complete computational species homology data:
    Anti-DFFA (ARP30359_T100)
    Peptide Sequence:
    Synthetic peptide located within the following region: MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDIL
    Product Format:
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
    Reconstitution and Storage:
    For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
    Concentration:
    Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
    Protein Interactions:
    DFFB; UBC; TUBB; ATG3; ISOC1; BZW2; MAT2B; FERMT2; TUBA1A; CNBP; RPS6KA3; PPP5C; PFAS; MAT2A; EIF6; GAPDH; EIF5; CTPS1; CKB; GZMB; CASP7; CASP3; GZMM; TOM1L2; SEPT5; YWHAZ; YWHAG; PRKAA2; STRN4; NECAB2; CIDEB; EWSR1; NAP1L5; TMBIM4; USP15; HERC1; MCC; BAK
    Blocking Peptide:
    For anti-DFFA (ARP30359_T100) antibody is Catalog # AAP30359 (Previous Catalog # AAPS08806)
    Datasheets / Downloads:
    Printable datasheet for anti-DFFA (ARP30359_T100) antibody
    Subunit:
    alpha
    Target Reference:
    Yan,B., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1504-1509

    Product Protocols: DFFA antibody tested with Human Jurkat Cells (ARP30359_T100)

    Aviva Systems Biology is the original manufacturer of this DFFA antibody (ARP30359_T100)

    Click here to view the DFFA antibody Western Blot Protocol

    Product Datasheet Link: DFFA antibody (ARP30359_T100)

    WB Suggested Anti-DFFA Antibody Titration: 5.0ug/ml
    ELISA Titer: 1:312500
    Positive Control: Jurkat

    Western Blot image:


    Description of Target: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

    Questions pertaining to this data can be directed to techsupport@avivasysbio.com

    Aviva Systems Biology’s DFFA antibody (ARP30359_T100) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

    To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

    All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

     
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