Catalog No: ARP30359_T100
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ARP30359_T100
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Datasheets/ManualsPrintable datasheet for anti-DFFA (ARP30359_T100) antibody
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman, Mouse, Rat, Cow, Dog, Guinea Pig, Horse
Product FormatLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ClonalityPolyclonal
HostRabbit
ApplicationWB
Reconstitution and StorageFor short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
ImmunogenThe immunogen is a synthetic peptide directed towards the N terminal region of human DFFA
PurificationProtein A purified
Predicted Homology Based on Immunogen SequenceCow: 92%; Dog: 83%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 83%; Rat: 92%
Peptide SequenceSynthetic peptide located within the following region: MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDIL
Concentration1.0 mg/ml
Blocking PeptideFor anti-DFFA (ARP30359_T100) antibody is Catalog # AAP30359 (Previous Catalog # AAPS08806)
Subunitalpha
ReferenceYan,B., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1504-1509
Gene SymbolDFFA
Gene Full NameDNA fragmentation factor, 45kDa, alpha polypeptide
Alias SymbolsDFF1, ICAD, DFF-45
NCBI Gene Id1676
Protein NameDNA fragmentation factor subunit alpha
Description of TargetApoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Uniprot IDO00273
Protein Accession #NP_004392
Nucleotide Accession #NM_004401
Protein Size (# AA)331
Molecular Weight37kDa
Protein InteractionsDFFB; UBC; TUBB; ATG3; ISOC1; BZW2; MAT2B; FERMT2; TUBA1A; CNBP; RPS6KA3; PPP5C; PFAS; MAT2A; EIF6; GAPDH; EIF5; CTPS1; CKB; GZMB; CASP7; CASP3; GZMM; TOM1L2; SEPT5; YWHAZ; YWHAG; PRKAA2; STRN4; NECAB2; CIDEB; EWSR1; NAP1L5; TMBIM4; USP15; HERC1; MCC; BAK
  1. What is the species homology for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse".

  2. How long will it take to receive "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".

  3. What buffer format is "DFFA Antibody - N-terminal region (ARP30359_T100)" provided in?

    This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    This target may also be called "DFF1, ICAD, DFF-45" in publications.

  5. What is the shipping cost for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "37kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "DFFA Antibody - N-terminal region (ARP30359_T100)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "DFFA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "DFFA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "DFFA"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "DFFA"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "DFFA"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "DFFA"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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