GRIN2B Antibody - middle region (ARP35015_P050)

Catalog No: ARP35015_P050

Click here to learn more about Aviva's By-Request Conjugation Service.

WB Suggested Anti-GRIN2B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human brain

Sample Type:
Rat Hippocampal Neurons - 14DIV
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-Cy3
Secondary Antibody Dilution:
1:500
Color/Signal Descriptions:
Green: GFP Red: NR2b Yellow: VGLUT12
Gene Name:
GRIN2B
Submitted by:
Dan Fowler - University of Oregon, Institute of Neuroscience

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GRIN2B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: RSPDHKRYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKR
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-GRIN2B (ARP35015_P050) antibody is Catalog # AAP35015 (Previous Catalog # AAPP06242)
• Subunit: epsilon-2
• Reference: Cais,O., (2008) Neuroscience 151 (2), 428-438

Target Info

• Gene Symbol: GRIN2B
• Gene Full Name: Glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Alias Symbols: NR3, MRD6, NR2B, hNR3, DEE27, EIEE27, GluN2B, NMDAR2B
• NCBI Gene Id: 2904
• Protein Name: Glutamate [NMDA] receptor subunit epsilon-2
• Description of Target: GRIN2B is the NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium.N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. Sequence Note: This RefSeq was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-544 U90278.1 31-574 545-545 U88963.1 545-545 546-3677 U90278.1 576-3707 3678-5941 AC007535.3 145452-147715
• Uniprot ID: Q13224
• Protein Accession #: NP_000825
• Nucleotide Accession #: NM_000834
• Protein Size (# AA): 1484
• Molecular Weight: 163kDa
• Protein Interactions: SPTAN1; PRKCA; FYN; CAMK2G; DLG4; DLG1; KDM5B; TANC1; SYNGAP1; GRIN1; CAMK2N1; ARHGAP32; EXOC4; CAMK2B; MAGI3; LIN7B; CAMK2D; GRIN3A; CAMK2A; DLG2; AP1M1; LIN7A; INADL; IL16; PARK2; AP4M1; AP2M1; EPHB2; DLG3; SRC; GNB2L1; PRKCG; PRKCB; PTPN11; PLCG1; PIK3