FAS antibody - middle region (ARP30627_P050)

Catalog#: ARP30627_P050

• Size: 50ug

Human Liver

• Description of Target: FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
• Gene Symbol: FAS
• Official Gene Full Name: Fas (TNF receptor superfamily, member 6)
• Alias Symbols: ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6
• Protein Accession#: NP_000034
• Nucleotide Accession#: NM_000043
• Swissprot Id: P25445
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 335
• Molecular Weight: 36kDa
• Application: WB
• Partner Proteins: CTNNB1, KAT5, RUVBL2, TP63, SUMO1, ANK3, APAF1, BTK, C14orf1, CALM1, CASP8, CASP8AP2, CD47, CRMP1, DAXX, EEF1A1, EGFR, EZR, FADD, FAF1, FAIM2, FAS, FASLG, FBF1, FEM1B, FYN, HIPK3, LCK, LRIF1, MET, PDCD6, PRKCA, PTPN13, PTPN6, RAP1A, RIPK1, SUMO1, TNFSF13, TRADD, UBA7, UBE2I, ARHGDIA, BID, BRE, C14orf1, CASP10, CASP8, CASP8AP2, CFLAR, CRMP1, DAXX, EEF1A1, EZR, FADD, FADD, FADD, FAF1, FAIM2, FAS, FASLG, FBF1, FYN, HIPK3, LCK, LRIF1, MAPK8, MSN, NOL3, NOL3, PDCD6, RHOA, RIPK1, SUMO1, TNF, TNFRSF10B, TNFRSF1A, TNFSF13, UBE2I
• Immunogen: The immunogen for anti-FAS antibody: synthetic peptide directed towards the middle region of human FAS
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: Synthetic peptide located within the following region: KKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV
• Blocking Peptide: For anti-FAS antibody is Catalog # AAP30627 (Previous Catalog # AAPP01280)
• Key Reference: N/A
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-FAS antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: FAS antibody tested with Human Fetal Liver Tissue (ARP30627_P050)

Aviva Systems Biology is the original manufacturer of this FAS antibody (ARP30627_P050)

Click here to view the FAS antibody Western Blot Protocol

Product Datasheet Link: FAS antibody (ARP30627_P050)

WB Suggested Anti-FAS Antibody Titration: 0.2-1 ug/ml
Positive Control: Fetal Liver

Western Blot image:


Description of Target: FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s FAS antibody (ARP30627_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com