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CHEK2 antibody - N-terminal region (ARP30290_P050)

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Description of Target:
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Gene Symbol:
CHEK2
Official Gene Full Name:
Checkpoint kinase 2
NCBI Gene Id:
11200
Alias Symbols:
RP11-436C9.1; CDS1; CHK2; HuCds1; LFS2; PP1425; RAD53; hCds1
Sample Type Confirmation:

CHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat

Tissue Tool:
Find tissues and cell lines supported to express CHEK2.
Protein Accession #:
NP_665861
Nucleotide Accession#:
NM_145862
Swissprot Id:
O96017-12
Host:
Rabbit
Clonality:
Polyclonal
Protein Name:
Serine/threonine-protein kinase Chk2
Protein Size (# AA):
514
Molecular Weight:
58kDa
Application:
IHC, WB
Partner Proteins:
E2F1, E2F4, E2F5, CHUK, ESR1, FUBP1, NCOA3, ORC2L, POLR2A, XRCC5, BRCA1, CDK4, CDKN1A, CDKN1A, CDKN1A, CDKN2A, CSNK2A1, DDB2, DNMT3A, FBXW7, FBXW7, KAT2A, MAPK3, MAX, MIR17HG, MLH1, MYCBP2, NCL, NFYB, NFYC, NMI, NPM1, PARP10, PMAIP1, PML, RIBIN, RRM2B, SP1, SP1, TAF1A, TAF1B, TAF1C, TBP, TRRAP, ACT
Immunogen:
The immunogen for anti-CHEK2 antibody: synthetic peptide directed towards the N terminal of human CHEK2
Product Format:
Lyophilized powder
Purification:
Affinity Purified
Predicted Homology Based on Immunogen Sequence:
Human: 100%
Species Reactivity:
Human
Datasheets / Downloads:
Printable datasheet for
anti-CHEK2 antibody
- ARP30290_P050
Peptide Sequence:
Synthetic peptide located within the following region: MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
Blocking Peptide:
For anti-CHEK2 antibody is Catalog # AAP30290 (Previous Catalog # AAPS08701)
Key Reference:
Kobayashi,A., (2006) Histol. Histopathol. 21 (4), 373-382
Reconstitution and Storage:
Add 50 ul of distilled water. Final anti-CHEK2 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles.
 
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