IBSP antibody - N-terminal region (ARP36681_T100)

Catalog#: ARP36681_T100

• Size: 100ug

Human HepG2

• Description of Target: The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
• Gene Symbol: IBSP
• Official Gene Full Name: Integrin-binding sialoprotein
• Alias Symbols: BSP; BNSP; SP-II; BSP-II
• Protein Accession#: NP_004958
• Nucleotide Accession#: NM_004967
• Swissprot Id: P21815
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 317
• Molecular Weight: 35kDa
• Application: WB
• Immunogen: The immunogen for anti-IBSP antibody: synthetic peptide directed towards the N terminal of human IBSP
• Product Format: Lyophilized powder
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Human:100%; Vibrio cholerae MZO-2:90%; 
• Peptide Sequence: Synthetic peptide located within the following region: SATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEE
• Blocking Peptide: For anti-IBSP antibody is Catalog # AAP36681 (Previous Catalog # AAPP08108)
• Key Reference: Barnes,G.L., et al., (2003) Cancer Res. 63 (10), 2631-2637
• Reconstitution and Storage: Add 100 μl of distilled water. Final anti-IBSP antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: IBSP antibody tested with Human Hepg2 Cells (ARP36681_T100)

Aviva Systems Biology is the original manufacturer of this IBSP antibody (ARP36681_T100)

Click here to view the IBSP antibody Western Blot Protocol

Product Datasheet Link: IBSP antibody (ARP36681_T100)

WB Suggested Anti-IBSP Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2

Western Blot image:


Description of Target: The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s IBSP antibody (ARP36681_T100) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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