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Datasheets/Manuals | Printable datasheet for Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer |
Clonality | Monoclonal |
Clone | A8A8 |
Isotype | IgG1 |
Host | Mouse |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex. |
:: | Positive Control: Jurkat Cell lysate |
:: | Cellular Location: Cytoplasmic |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein. This sequence is identical among human, mouse, bovine and dog. |
Purification | The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping |
Concentration | 100ug/200ul |
Target Post-Translational Modification | Phosphorylated |
Specificity | IRAK4 |
Formulation | This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Gene Symbol | IRAK4 |
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Gene Full Name | interleukin 1 receptor associated kinase 4 |
Alias Symbols | IMD67;interleukin-1 receptor-associated kinase 4;IPD1;IRAK-4;NY-REN-64;REN64;renal carcinoma antigen NY-REN-64. |
NCBI Gene Id | 51135 |
Description of Target | IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro sμggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex. |
Uniprot ID | Q9NWZ3 |
Molecular Weight | 51 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse".
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How long will it take to receive "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
This target may also be called "IMD67;interleukin-1 receptor-associated kinase 4;IPD1;IRAK-4;NY-REN-64;REN64;renal carcinoma antigen NY-REN-64." in publications.
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What is the shipping cost for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "51".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-IRAK-4 Antibody (Phospho-Thr345) (OAAI00415)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IRAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IRAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IRAK4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IRAK4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IRAK4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IRAK4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.