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Datasheets/Manuals | Printable datasheet for anti-Sptan1 (OAPC00143) antibody |
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Predicted Species Reactivity | Human, Rat, Cow |
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Product Format | Liquid. 100 ul in PBS + 10 mM sodium azide |
Clonality | Monoclonal |
Clone | 3D7 |
Isotype | IgG1 |
Host | Mouse |
Application | WB, IF |
Additional Information | Packaging : 100 ul in PBS + 10 mM sodium azide |
Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles. |
Immunogen | Recombinant construct containing the seventh, eighth and ninth repeats of human alpha II spectrin expressed in and purified from E. Coli. |
Purification | Affinity purified from tissue culture supernatant. |
Predicted Homology Based on Immunogen Sequence | Cow; Human; Rat |
Specificity | Specific for the ~240 k alpha II spectrin protein. |
Application Info | The antibody has been directly tested for reactivity in bovine, human and rat. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240k alpha II spectrin protein. Immunofluorescence of cultured rat neurons and glia showing axonal and dendritic staining of alpha II spectrin (green) revealing the submembraneous cytoskeleton and DNA (blue). |
Reference | Bennett V & Baines AJ.(2001) Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiol Rev. 81:1353-92. Eber S & Lux SE. (2004) Hereditary spherocytosis--defects in proteins that connect the membrane skeleton to the lipid bilayer. Semin Hematol 41:118-41. |
Gene Symbol | Sptan1 |
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Gene Full Name | Spectrin alpha chain, non-erythrocytic 1 |
Alias Symbols | A2a, IPF, Spna2 |
NCBI Gene Id | 64159 |
Protein Name | Spectrin alpha chain, non-erythrocytic 1 |
Description of Target | The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (alpha 1 and alpha 2) and five beta genes (beta 1- beta 5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004). |
Uniprot ID | P16086 |
Protein Accession # | NP_741984.2 |
Nucleotide Accession # | NM_171983.2 |
Molecular Weight | ~240kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Sptan1 Antibody (OAPC00143)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Rat, Cow".
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How long will it take to receive "Sptan1 Antibody (OAPC00143)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Sptan1 Antibody (OAPC00143)" provided in?
This item is provided in "Liquid. 100 ul in PBS + 10 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Sptan1 Antibody (OAPC00143)"?
This target may also be called "A2a, IPF, Spna2" in publications.
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What is the shipping cost for "Sptan1 Antibody (OAPC00143)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Sptan1 Antibody (OAPC00143)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Sptan1 Antibody (OAPC00143)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~240kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Sptan1 Antibody (OAPC00143)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SPTAN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SPTAN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SPTAN1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SPTAN1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SPTAN1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SPTAN1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.