Anti-Syntaxin 1A (OAPC00068)

Catalog No: OAPC00068

Size:100 ul

Western blot of rat cortex lysate showing specific immunolabeling of the ~ 36k syntaxin 1A protein.

Product Info

• Predicted Species Reactivity: Human, Rat, Pig
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Clone: SP8
• Isotype: IgG¹
• Host: Mouse
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least one year.
• Immunogen: Synaptic immunoprecipitate (crude) from human brain.
• Purification: Protein G purified culture supernatant.
• Predicted Homology Based on Immunogen Sequence: Human; Pig; Rat
• Specificity: The antibody is specific for the ~ 36 kDa syntaxin protein in Western blots of rat brain lysate. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. Does not work on paraffin sections.
• Application Info: The antibody has been directly tested for reactivity in human, rat, pig, and hamster.
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 36k syntaxin 1A protein.
  
• Reference: Khvotchev M, Dulubova I, Sun J, Dai H, Rizo J, Südhof TC (2007) Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus. J Neurosci. 2007 Nov 7;27(45):12147-55
  Castillo MA, Ghose S, Tamminga CA, Ulery-Reynolds PG (2010) Deficits in syntaxin 1 phosphorylation in schizophrenia prefrontal cortex. Biol Psychiatry. 67(3):208-16
  Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Research 609, 9-20 (1993).

Target Info

• Gene Symbol: STX1A
• Alias Symbols: STX1, HPC-1, P35-1, SYN1A
• NCBI Gene Id: 6804
• Protein Name: Syntaxin-1A
• Description of Target: Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).
• Uniprot ID: Q16623
• Protein Accession #: NP_001159375.1
• Molecular Weight: ~36kDa