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Datasheets/Manuals | Printable datasheet for OAPC00065 |
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Predicted Species Reactivity | Rat |
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Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Clonality | Monoclonal |
Isotype | IgG |
Host | Mouse |
Application | WB, IHC |
Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Reconstitution and Storage | For long term storage -20C is recommended. Stable at -20C for at least 1 year |
Immunogen | Recombinant protein corresponding to amino acid residues from the C-terminal region of rat synaptojanin 1. |
Purification | Protein G purified culture supernatant. |
Predicted Homology Based on Immunogen Sequence | Rat |
Specificity | Specific for the ~145k synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody. |
Application Info | The antibody has been directly tested for reactivity in Western blots with rat tissue. Does not cross react with mouse. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145k synaptojanin I protein. |
Reference | Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties (1996). Ramjaun AR, McPherson PS. J Biol Chem. Oct 4;271(40):24856-61. Synaptojanin 1-linked phosphoinositide dyshomeostasis and cognitive deficits in mouse models of Down's syndrome (2008).Voronov SV, Frere SG, Giovedi S, Pollina EA, Borel C, Zhang H, Schmidt C, Akeson EC, Wenk MR, Cimasoni L, Arancio O, Davisson MT, Antonarakis SE, Gardiner K, De Camilli P, Di Paolo G. Proc Natl Acad Sci U S A. Jul 8;105(27):9415-20. |
Gene Symbol | SYNJ1 |
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NCBI Gene Id | 85238 |
Protein Name | Synaptojanin-1 |
Description of Target | Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down's syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5-bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008). |
Uniprot ID | Q62910 |
Protein Accession # | NP_445928.2 |
Molecular Weight | ~145kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Synaptojanin I (OAPC00065)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".
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How long will it take to receive "Anti-Synaptojanin I (OAPC00065)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Synaptojanin I (OAPC00065)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Synaptojanin I (OAPC00065)"?
This target may also be called "" in publications.
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What is the shipping cost for "Anti-Synaptojanin I (OAPC00065)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Synaptojanin I (OAPC00065)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Synaptojanin I (OAPC00065)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~145kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Synaptojanin I (OAPC00065)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SYNJ1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SYNJ1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SYNJ1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SYNJ1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SYNJ1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SYNJ1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.