- Description of Target:
- NLK has a role in cell fate determination, required for differentiation of bone marrow stromal cells. NLK acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. NLK may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP By similarity.NLK is involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
- Gene Symbol:
- Official Gene Full Name:
- Nemo-like kinase
- NCBI Gene Id:
- Alias Symbols:
- DKFZp761G1211; FLJ21033
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express NLK.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express NLK.
- Swissprot Id:
- Protein Accession #:
- Nucleotide Accession #:
- Protein Name:
- Serine/threonine-protein kinase NLK
- Protein Size (# AA):
- Molecular Weight:
- Protein Interactions:
- KRTAP5-6; KRTAP10-3; KRTAP4-2; QRICH1; ZHX3; KRTAP5-9; GRN; TP53; MDM2; STAT5A; LEF1; CNOT2; SMAD4; KPNB1; BACH1; TNKS1BP1; FAM222A; C2orf44; CEP97; RNF219; FAM222B; CNOT11; NLK; PASK; SHANK2; UBAP2L; CCP110; TRPS1; TLE3; SKI; RANGAP1; RANBP2; PKM; CNOT3;
- The immunogen is a synthetic peptide directed towards the middle region of human NLK
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Affinity Purified
- Complete computational species homology data:
- Anti-NLK (ARP56863_P050)
- Predicted Homology Based on Immunogen Sequence:
- Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
- Species Reactivity:
- Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rabbit; Rat; Zebrafish
- Datasheets / Downloads:
- Printable datasheet for anti-NLK (ARP56863_P050) antibody
- Peptide Sequence:
- Synthetic peptide located within the following region: RLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEI
- Blocking Peptide:
- For anti-NLK (ARP56863_P050) antibody is Catalog # AAP56863 (Previous Catalog # AAPP44229)
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Aviva Systems Biology is the original manufacturer of this NLK antibody (ARP56863_P050)
Click here to view the NLK antibody Western Blot Protocol
Product Datasheet Link: NLK antibody (ARP56863_P050)
WB Suggested Anti-NLK Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T
Western Blot image:
Description of Target: NLK has a role in cell fate determination, required for differentiation of bone marrow stromal cells. NLK acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. NLK may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP By similarity.NLK is involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
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