TSLP ELISA Kit (Human) : 96 Wells (OKAA00028-96W)

Catalog No: OKAA00028-96W

Size:96 Wells

Standard curve of TSLP protein using OKAA00028_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human VEGF Standard lyophilized, 2000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human VEGF antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human TSLP (8x12)
  2. 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 2 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human TNF-alpha. The factors listed below were prepared at 70ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: TNF-b, GM-CSF, s TNF RI, s TNF RII, TNF-b
  Recombinant mouse: TNF-alpha, TNF-alpha（truncated）, s TNF RI
  Recombinant porcine: TNF-alpha
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TSLP
• Gene Full Name: thymic stromal lymphopoietin
• Alias Symbols: Thymic stromal lymphopoietin
• NCBI Gene Id: 85480
• Protein Name: Thymic stromal lymphopoietin
• Description of Target: This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants.
• Uniprot ID: Q969D9
• Protein Accession #: NP_149024.1
• Nucleotide Accession #: NM_033035.4