Catalog No: OKEH00149
Size:96T
Price: $425.00
SKU
OKEH00149
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LEPR ELISA Kit (Human) : 96 Wells (OKEH00149)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LEPR ELISA Kit (Human) (OKEH00149) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEPR captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 24)
ELISA Kit Component
ComponentAmount
LEPR Microplate96 Wells (12 x 8 Well strips)
LEPR Lyophilized Standard2
100X Biotinylated LEPR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.32 ng/mL
SpecificityNatural and recombinant Human Leptin receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLEPR
Gene Full Nameleptin receptor
Alias SymbolsCD295;huB219;LEP-R;LEPRD;leptin receptor;OB receptor;OBR;OB-R.
NCBI Gene Id3953
Protein NameP48357
Description of TargetLeptin receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P48357
Protein Accession #NP_001003679
Nucleotide Accession #NM_001003679
Protein Size (# AA)896
Molecular Weight102 kDa
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