LEFTY2 ELISA Kit (Mouse) (OKCD00192)

Catalog No: OKCD00192 (Formerly GWB-KBBOP1)

Size:96WELLS

Mouse Lefty2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-92%	89-104%	79-91%	82-91%
  EDTA plasma(n=5)	93-105%	79-97%	96-105%	96-103%
  heparin plasma(n=5)	98-105%	78-103%	88-101%	94-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 2 (LEFTY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 2 (LEFTY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Left/Right Determination Factor 2 (LEFTY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-93	90
  EDTA plasma(n=5)	79-91	83
  heparin plasma(n=5)	80-99	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Lefty2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Lefty2 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Lefty2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 5.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 2 (LEFTY2). No significant cross-reactivity or interference between Left/Right Determination Factor 2 (LEFTY2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Lefty2
• Gene Full Name: left-right determination factor 2
• Alias Symbols: 6030463A22Rik;Eba;Ebaf;endometrial bleeding associated factor;Left;Lefta;Leftb;left-right determination factor 2;left-right determination factor B;left-right determination, factor A;lefty-2;lefty-B;Protein lefty-2;Protein lefty-B.
• NCBI Gene Id: 320202
• Protein Name: Left-right determination factor 2
• Description of Target: Required for left-right asymmetry determination of organ systems in mammals.
• Uniprot ID: P57785
• Protein Accession #: NP_796073
• Nucleotide Accession #: NM_177099.4
• Protein Size (# AA): 368