LCP1 ELISA Kit (Human) (OKCD00336)

Catalog No: OKCD00336 (Formerly GWB-KBBJV8)

Size:96WELLS

Human LCP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Plastin (LCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L-Plastin (LCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L-Plastin (LCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of L-Plastin (LCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level L-Plastin (LCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level L-Plastin (LCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LCP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • LCP1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated LCP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphocyte Cytosolic Protein 1 (LCP1). No significant cross-reactivity or interference between Lymphocyte Cytosolic Protein 1 (LCP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LCP1
• Gene Full Name: lymphocyte cytosolic protein 1
• Alias Symbols: bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin));CP64;epididymis secretory protein Li 37;HEL-S-37;LC64P;LCP-1;LPL;L-PLASTIN;L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P);Lymphocyte cytosolic protein 1;Lymphocyte cytosolic protein-1 (plasmin);plastin-2;PLS2.
• NCBI Gene Id: 3936
• Protein Name: Plastin-2
• Description of Target: Actin-binding protein (PubMed:16636079, PubMed:17294403, PubMed:28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed:17294403).
• Uniprot ID: P13796
• Protein Accession #: NP_002289
• Nucleotide Accession #: NM_002298.4
• Protein Size (# AA): 627