LBP ELISA Kit (Mouse) (OKBB00573)

Catalog No: OKBB00573

Size:96 Wells

Mouse LBP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LBP has been precoated onto 96-well plates. Standards(NS0, G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse LBP amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	5.61	33.8	68.2	5.32	36.5	74.9
  SD	0.32	1.55	3.41	0.46	1.93	4.81
  CV%	5.7	4.6	5	8.6	5.3	6.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse LBP Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse LBP Standard: 2 x 100 ng/tube
  • 100X Biotinylated Anti-Mouse LBP Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant mouse LBP
• Publications:

  Integrative analysis of gut microbiota composition, host colonic gene expression and intraluminal metabolites in aging C57BL/6J mice. Aging (Albany NY). 10, 930-950 (2018). 29769431

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G25-V481
• Description:
  View product citations for LBP ELISA Kit (Mouse) (OKBB00573) on CiteAb

Target Info

• Gene Symbol: LBP
• Gene Full Name: lipopolysaccharide binding protein
• Alias Symbols: BPI fold containing family D, member 2, Bpifd2, LBP, LBP_HUMAN, LBP1, Lipopolysaccharide binding protein, Lipopolysaccharide-binding protein, LPS binding protein, Ly88, MGC22233, OTTHUMP00000030965, RP23-407H16.4
• NCBI Gene Id: 16803
• Protein Name: Lipopolysaccharide-binding protein
• Description of Target: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
• Uniprot ID: Q61805
• Protein Accession #: NP_032515.2
• Nucleotide Accession #: NM_008489.2