Catalog No: OKCD00580 (Formerly GWB-KBBYI4)
Size:96WELLS
Price: $675.00
SKU
OKCD00580
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Datasheets/ManualsPrintable datasheet for LASP1 ELISA Kit (Human) (OKCD00580)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And SH3 Protein 1 (LASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And SH3 Protein 1 (LASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And SH3 Protein 1 (LASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LIM And SH3 Protein 1 (LASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LASP1 Microplate96 Wells (12 x 8 Well Strips)
LASP1 Lyophilized Standard2 x 10 ng
100X Biotinylated LASP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of LIM And SH3 Protein 1 (LASP1). No significant cross-reactivity or interference between LIM And SH3 Protein 1 (LASP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLASP1
Gene Full NameLIM and SH3 protein 1
Alias SymbolsLasp-1;LIM and SH3 domain protein 1;metastatic lymph node gene 50 protein;MLN50.
NCBI Gene Id3927
Protein NameLIM and SH3 domain protein 1
Description of TargetPlays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
Uniprot IDQ14847
Protein Accession #NP_001258537
Nucleotide Accession #NM_001271608.1
Protein Size (# AA)261
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