JTB ELISA Kit (Human) (OKCD02022)

Catalog No: OKCD02022 (Formerly GWB-KBBFB5)

Size:96WELLS

Human JTB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jumping Translocation Breakpoint Protein (JTB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Jumping Translocation Breakpoint Protein (JTB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jumping Translocation Breakpoint Protein (JTB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Jumping Translocation Breakpoint Protein (JTB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Jumping Translocation Breakpoint Protein (JTB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Jumping Translocation Breakpoint Protein (JTB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-JTB Microplate: 96 Wells (12 x 8 Well Strips)
  • JTB Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated JTB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Jumping Translocation Breakpoint Protein (JTB). No significant cross-reactivity or interference between Jumping Translocation Breakpoint Protein (JTB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: JTB
• Gene Full Name: Jumping translocation breakpoint
• Alias Symbols: PAR, hJT, HJTB, HSPC222
• NCBI Gene Id: 10899
• Protein Name: Protein JTB
• Description of Target: Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential.
• Uniprot ID: O76095
• Protein Accession #: NP_006685.1
• Nucleotide Accession #: NM_006694.3
• Protein Size (# AA): 146