HGF ELISA Kit (Rat) (OKBB00592)

Catalog No: OKBB00592

Size:96 Wells

Rat HGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's rat HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HGF has been precoated onto 96-well plates. Standards(NS0, Q33-R495(α) & V496-L728(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat HGF amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1765	3081	6143	1860	3542	6496
  SD	74.13	141.7	344	126.5	180.6	487.2
  CV%	4.2	4.6	5.6	6.8	5.1	7.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Rat HGF Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Rat HGF Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Rat HGF Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (EDTA, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant rat HGF
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q33-R495 (alpha) & V496-L728 (beta)
• Description: For quantitative detection of rat HGF in cell culture supernatants, serum and plasma(EDTA, citrate).

Target Info

• Gene Symbol: HGF
• Gene Full Name: hepatocyte growth factor (hepapoietin A; scatter factor)
• Alias Symbols: DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepapoietin A, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A scatter factor, Hepatocyte growth factor beta chain, Hepatopoietin-A, Hgf, HGF_HUMAN, HGFB, HPTA, Lung fibroblast derived mitogen, Scatter factor, SF
• NCBI Gene Id: 24446
• Protein Name: Hepatocyte growth factor
• Description of Target: Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.
• Uniprot ID: P17945
• Protein Accession #: NP_058713.1
• Nucleotide Accession #: NM_017017.2