Catalog No: OKCA00094
Size:96 Wells
Price: $475.00
SKU
OKCA00094
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Hemoglobin ELISA Kit (Human) (OKCA00094) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Hemoglobin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hemoglobin and incubated. The Hemoglobin found in the sample or standards competes with the biotinylated Hemoglobin for limited binding sites on the immobilized anti-human Hemoglobin antibody. Excess unbound biotinylated Hemoglobin and sample or standard Hemoglobin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hemoglobin captured in the well and inversely proportional to the amount of Hemoglobin which was contained in the sample or standard. | ||||||||||||||||||||||||
ELISA Kit Range | 15.6 ng/mL - 1,000 ng/mL | ||||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20) | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, Plasma, Lysate for RBC | ||||||||||||||||||||||||
Sensitivity | 7.8 ng/ml | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of human Hb. No significant cross-reactivity or interference between human Hb and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Immunoassay |
Gene Symbol | Hemoglobin |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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