EPO ELISA Kit (Rat) (OKBB00617)

Catalog No: OKBB00617

Size:96 Wells

Rat EPO ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's rat EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(E.coli, T44-K226) and test samples are added to the wells, a biotinylated detection monoclonal antibody from rat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat EPO amount of sample captured in plate.
• ELISA Kit Range: 46.9 pg/ml - 3,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	513	1265	1974	648	1450	2301
  SD	18.98	53.13	96.73	33.7	82.65	145
  CV%	3.7	4.2	4.9	5.2	5.7	6.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Rat EPO Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Rat EPO Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Rat EPO Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin) and tissue homogenates
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant rat EPO
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: T44-K226
• Description: For quantitative detection of rat EPO in cell culture supernatants, serum and plasma(heparin).

Target Info

• Gene Symbol: EPO
• Gene Full Name: erythropoietin
• Alias Symbols: EP, EPO, EPO_HUMAN, Epoetin, Erythropoietin, MVCD2
• NCBI Gene Id: 24335
• Protein Name: Erythropoietin
• Description of Target: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.
• Uniprot ID: P29676
• Protein Accession #: NP_058697.1
• Nucleotide Accession #: NM_017001.1