Catalog No: OKBB00641
Size:96 Wells
Price: $583.00
SKU
OKBB00641
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been precoated onto 96-well plates. Standards(NS0, S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human EPCR amount of sample captured in plate.
ELISA Kit Range312 pg/ml - 20,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)2.218.4514.42.138.7913.7
SD0.0950.3130.6910.1240.4661.04
CV%4.33.74.85.85.37.6
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human EPCR Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human EPCR Standard2 x 20 ng/tube
100X Biotinylated Anti-Human EPCR Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human EPCR
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: S18-S210
DescriptionFor quantitative detection of activated human EPCR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolPROCR
Gene Full Nameprotein C receptor, endothelial
Alias SymbolsCD201, EPCR, PROCR, Q9UNN8
NCBI Gene Id10544
Protein NameEndothelial protein C receptor
Description of TargetEndothelial protein C receptor (EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
Uniprot IDQ9UNN8
Protein Accession #NP_006395.2
Nucleotide Accession #NM_006404.4
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