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IL1A ELISA Kit (Human) : 48 Wells (OKAA00007_48W)

  • Catalog#: OKAA00007_48W
  • 2 weeks

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48 Wells
$185.00
In Stock
Application:
ELISA
Gene Symbol:
IL1A
Official Gene Full Name:
interleukin 1 alpha
NCBI Gene Id:
3552
Alias Symbols:
IL1, IL-1A, IL1F1, IL1-ALPHA
Swissprot Id:
P01583
Protein Accession #:
NP_000566
Nucleotide Accession #:
NM_000575
Species Reactivity:
Human
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
4 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Description of Target:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL1A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL1A.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets / Downloads:
OKAA00007_48W
Specificity:
This assay recognizes both natural and recombinant human IFN-gamma. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

Recombinant human: IL-6, IL-8, TNF-alpha, TNF-b, VEGF

Recombinant mouse: IL-1a, IFN-gamma

Recombinant porcine: IL-1b, IFN-gamma
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-1?(8x12)
2. 2 vials human IL-1? Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-1? monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Protein Name:
interleukin-1 alpha
Protocol:
Tips Information:
 
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