Catalog No: OKAG00118 (Formerly GWB-SKR118)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00118
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human EGFR ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human EGFR concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope onHuman EGFRwhile the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range32-2000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate12 x 8-WellMicrostrips
Protein StandardLyophilized
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30 μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate6 mL
Stop Solution12 mL
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity32 pg/mL
SpecificityThe Human EGF-R ELISA is capable of recognizing both recombinant and naturally produced Human EGF-R proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 3% cross reactivity.
• Murine: EGF-R
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: EGF, ErbB2, ErbB3
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolEGFR
Gene Full Nameepidermal growth factor receptor
Alias Symbolsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor tyrosine kinase domain;ERBB;ERBB1;erb-b2 receptor tyrosine kinase 1;ERRP;HER1;mENA;NISBD2;PIG61;proto-oncogene c-ErbB-1;receptor tyrosine-protein kinase erbB-1.
NCBI Gene Id1956
Protein NameP00533
Description of TargetEpidermal growth factor receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P00533
Protein Accession #NP_005219.2
Nucleotide Accession #NM_005228.3
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