- Description:
- 0
- Key Reference:
- 1. Aarvak, T. et al. (1999). J. Immunol. 162:1246- 51
2. Nakae, S. et al. (2003). PNAS. 100:5986- 5990
3. Fossiez, F. et al. (2004). Int. Rev. Immunol. 16:541- 51
4. Jovanovic, D.V. et al. (1998). J. Immunol. 160:3513- 21
5. Shalom- Barak, T. et al. (1998). J. Biol. Chem. 273:27467- 73
6. Schwarzenberger P. et al. (2000). J. Immunol. 164:4783- 9
7. Shahrara, S. et al. (2009). J. Immunol. 182:3884- 91
8. McKenzie, B.S. et al. (2005). Trends Immunol. 27:17- 23. - Datasheets / Downloads:
- OKSB00015
- Reconstitution and Storage:
- Cell Culture Supernatants - Remove particulates by centrifugation and assay immediately or aliquotand store samples at - 20 C. Avoid repeated freeze- thaw cycles.Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugationfor 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at - 20 C. Avoid repeated freeze- thaw cycles.Note: Serum samples require a 200- fold dilution. A suggested 200- fold dilution is 5.0uL sample into995uL Assay Diluent.
- Protocol:
- Tips Information:
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