PRMT2 Antibody - N-terminal region (ARP40196_T100)

Catalog No: ARP40196_T100

WB Suggested Anti-PRMT2 Antibody Titration: 0.625ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate

PRMT2 antibody - N-terminal region (ARP40196_T100) validated by WB using human LCL and mouse brains at 1:1000.

Rabbit Anti-PRMT2 Antibody
Catalog Number: ARP40196
Paraffin Embedded Tissue: Human Liver
Cellular Data: Liver cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-PRMT2 Antibody
Catalog Number: ARP40196
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ESQGEEPAECSEAGLLQEGVQPEEFVAIADYAATDETQLSFLRGEKILIL
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-PRMT2 (ARP40196_T100) antibody is Catalog # AAP40196 (Previous Catalog # AAPP22042)
• Reference: Qi,C., (2002) J. Biol. Chem. 277 (32), 28624-28630
• Publications:

  Arginine Methylation by PRMT2 Controls the Functions of the Actin Nucleator Cobl. Dev Cell. 45, 262-275.e8 (2018). 29689199

  Burton, A. et al. Single-cell profiling of epigenetic modifiers identifies PRDM14 as an inducer of cell fate in the mammalian embryo. Cell Rep. 5, 687-701 (2013). 24183668

  Spellman, C., Ahmed, M. M., Dubach, D. & Gardiner, K. J. Expression of trisomic proteins in Down syndrome model systems. Gene 512, 219-225 (2013). 23103828

  Zhong, J. et al. Identification and characterization of novel spliced variants of PRMT2 in breast carcinoma. FEBS J. 279, 316-35 (2012). 22093364

  Zhong, J. et al. Identification and expression analysis of a novel transcript of the human PRMT2 gene resulted from alternative polyadenylation in breast cancer. Gene 487, 1-9 (2011). 21820040

• Description:
  View product citations for PRMT2 Antibody - N-terminal region (ARP40196_T100) on CiteAb

Target Info

• Gene Symbol: PRMT2
• Gene Full Name: Protein arginine methyltransferase 2
• Alias Symbols: HRMT1L1
• NCBI Gene Id: 3275
• Protein Name: Protein arginine N-methyltransferase 2
• Description of Target: The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.
• Uniprot ID: P55345
• Protein Accession #: NP_001526
• Nucleotide Accession #: NM_001535
• Protein Size (# AA): 433
• Molecular Weight: 48kDa
• Protein Interactions: FIP1L1; SPAG8; RIPK2; BAG6; RB1; HNRNPA1; E2F1; UBC; PSMB3; HHV8GK18_gp81; CPSF7; DMRTB1; HNRNPUL1; PRMT2; THRB; PGR; NCOA6; NCOA1; ESR2; RXRA; PRRC2A; ESR1; PPARG; RARA;