PRM1 ELISA Kit (Human) (OKEH04849)

Catalog No: OKEH04849

Size:96T

Human PRM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology PRM1 ELISA Kit (Human) (OKEH04849) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRM1 captured in well.
• ELISA Kit Range: 1.56-100IU/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 4.1% (n = 20)
  Mean Inter-Assay CV: < 7.3% (n = 20)
• ELISA Kit Component:
  • PRM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • PRM1 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • PRM1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.098IU/mL
• Specificity: Natural and recombinant Human Sperm protamine P1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PRM1
• Gene Full Name: protamine 1
• Alias Symbols: cancer/testis antigen family 94, member 1;CT94.1;cysteine-rich protamine;P1;sperm protamine P1;testicular tissue protein Li 91;testis specific protamine 1.
• NCBI Gene Id: 5619
• Protein Name: P04553
• Description of Target: Sperm protamine P1
• Uniprot ID: https://www.uniprot.org/uniprot/P04553
• Protein Accession #: NP_002752.1
• Nucleotide Accession #: NM_002761.2
• Protein Size (# AA): 51
• Molecular Weight: 7 kDa