SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKEH03560
Size:96T
Price: $600.00
SKU
OKEH03560
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRL8A8 ELISA Kit (Mouse) (OKEH03560) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Prl8a8 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prl8a8 and incubated. The Prl8a8 found in the sample or standards competes with the biotinylated Prl8a8 for limited binding sites on the immobilized anti-human Prl8a8 antibody. Excess unbound biotinylated Prl8a8 and sample or standard Prl8a8 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prl8a8 captured in the well and inversely proportional to the amount of Prl8a8 which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.9% (n = 20)
Mean Inter-assay CV%: <= 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Prl8a8 Microplate96 Wells (12 x 8 Well strips)
Prl8a8 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Prl8a8-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Mouse Prolactin-8A8
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPrl8a8
Gene Full Nameprolactin family 8, subfamily a, member 81
Alias Symbols1600032B14Rik;Ghd14;growth hormone d14;placental prolactin-like protein C3;PLP-C;PLP-C gamma2;PLP-C3;PLP-Cgamma;Pr;PRL-like protein C3;Prlpc3;prolactin-8A8;prolactin-like protein C-gamma.
NCBI Gene Id74188
Protein NameQ9DAS4
Description of TargetProlactin-8A8
Uniprot IDhttps://www.uniprot.org/uniprot/Q9DAS4
Protein Accession #NP_001298054.1
Nucleotide Accession #NM_001311125.1
Protein Size (# AA)241
Molecular Weight28 kDa
Write Your Own Review
You're reviewing:PRL8A8 ELISA Kit (Mouse) (OKEH03560)
Your Rating